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Protein Facility

News

Seminar: Young-Jin Lee, March 27, 2009

April 14, 2008
The amino acid analysis service has been discontinued, see here for details.

Typhoon imaging system and the 2D gel documentation/analysis system

The facility has two methods for the acquisition of digital gel images. For the acquisition of Coomassie Blue stained gels, the Amersham Pharmacia Biotech ImageScanner flatbed scanner with transparency module can be used. Once the gel image has been digitized, 2-D gel analysis can be performed using PDQuest from Bio-Rad. This software allows the comparison of spots within 1 or more gels for differences in quantity, pI or molecular weight. Gels can also be compared for the presence or absence of proteins of interest. This can be useful in determining protein spots that may be candidates for further analysis.

The Typhoon 8600 from Amersham Pharmacia Biotech is a multimode image scanner that can be used for the acquisition of phosphorescence, fluorescence or chemiluminescent samples. The scanner is capable of imaging gel sandwiches, agarose and polyacrylamide gels, membranes, microplates and microarrays. Samples can also be labeled with multiple dyes and imaged on the Typhoon. After image acquisition, the image can be analyzed for quantitative data using ImageQuant software from Amersham Pharmacia Biotech.