Typhoon imaging system and the 2D gel documentation/analysis system
The facility has two methods for the acquisition of digital gel
images. For the acquisition of Coomassie Blue stained gels, the
Amersham Pharmacia Biotech
ImageScanner flatbed scanner with transparency module can be used.
Once the gel image has been digitized, 2-D gel analysis can be
performed using PDQuest from Bio-Rad.
This software allows the comparison of spots within 1 or more gels
for differences in quantity, pI or molecular weight. Gels can also
be compared for the presence or absence of proteins of interest.
This can be useful in determining protein spots that may be
candidates for further analysis.
The Typhoon 8600 from Amersham
Pharmacia Biotech is a multimode image scanner that can be used
for the acquisition of phosphorescence, fluorescence or
chemiluminescent samples. The scanner is capable of imaging gel
sandwiches, agarose and polyacrylamide gels, membranes, microplates
and microarrays. Samples can also be labeled with multiple dyes and
imaged on the Typhoon. After image acquisition, the image can be
analyzed for quantitative data using ImageQuant software from
Amersham Pharmacia Biotech.
