SEMINAR
Michael Merriman
Sr. Field Applications Specialist, Proteomics
Mass Spec & Chromatography Products
Applied Biosystems
"Quantifying Proteins
by Mass Spectrometry: Labeling Chemistries, Workflows and Analysis"
The talk will cover the basic chemistries
offered by Applied Biosystems, focussing on iTRAQ,with some information on ICAT
and SILAC reagents. Additionally, I will have a few slides which
demonstrate the iTRAQ chemistry and workflows applied to one or two biomarkers
applications run on 4800 or QTRAP instruments. I will then finish
with an overview of Protein Pilot, the software used to process the data
acquired on iTRAQ and SILAC samples by extracting fragmentation information,
calculating peaks areas and ratios for the reporter ions and presenting the
data in an easy to access format.
12:30 -1:30 p.m.
1420 Molecular Biology Building