LC/MS: Fundamentals and its Application in Proteomics and Bacteria Identification
October 14, 2003
11:00 am
1102 Molecular Biology Building
Fumin Li
Protein identification and quantification are the center of today's research since the genome has been sequenced. Mass spectrometry (LC/MS and LC/MS/MS) has become a universal tool in this area.
The fundamentals of mass spectrometry, especially quadrupole instrument, electrospray ionization and LC/MS will be covered. Basic knowledge of peptides, such as how a tryptic peptide fragments in collision cell, and nomenclature of the daughter ions will be addressed. Sample preparation, starting from 2D gel to the final mass spectrometry analysis, is extremely important for a successful analysis. Some general methods will be reviewed briefly. Subtopics of the seminar will include:
1) Analysis of intact protein and deconvolution of mass spectrum by software;
2) Analysis of peptide by LC/MS and calculation of its mass by software;
3) Protein sequencing by LC/MS/MS and database searching (SEQUEST 2.0);
4) Quantification of proteins and drugs (e.g. ICAT);
5) Characterization of phosphorylated proteins and peptides.
If time allows, identification of bacteria by ESI-MS and CE-MS, based on their specific biomarkers, will be discussed.
Hosted by the Protein Facility and the Ames Area Proteome Discussion Group.
For further information please contact Joel Nott or Chu-Xiong Liao at 294-3267 or Louisa Tabatabai at 294-6284.
protein@iastate.edu