Seminar Notice

The Protein Facility of the Office of Biotechnology and the Ames Area Proteomics Discussion Group will host two seminars by Dr. Tim Barder of Eprogen on Tuesday, August 24, 2004

"ProteoSep: A Viable All-Liquid Phase: Alternative to 2D Gels".
Dr. Tim Barder, Eprogen, Inc.

Talk I: 11:00 AM, 1102 Molecular Biology Building, Iowa State University Campus

Talk II: 3:00 PM, National Animal Disease Center, 2300 Dayton Avenue, Ames

ProteoSep™ is a novel protein mapping technology and a powerful way to analyze complex protein mixtures. ProteoSep is a gel-free, all-liquid phase alternative to 2D PAGE that uses standard HPLC instrument technology to produce high resolution 2D maps of complex protein systems such as whole cell lysates, plasma and sera. A unique high performance chromatofocusing (CF) column has been developed to provide the pI information biologists need in the first dimension analysis of complex protein systems. The use of HPLC provides these pI fractions in the liquid phase containing the intact proteins. Subsequent analysis of these pI fractions with proprietary reverse phase NPS® columns provides for the second dimension separation information based on hydrophobicity or MW, depending on whether you use UV or MS detection or both. A "2D protein map" is produced using ProteoVue™ Software, which provides either UV ­ pI or Mass ­ pI maps or both, displaying the proteins present in bands like that presented in a 2D PAGE gel. The all-liquid phase format provides for easy collection of sample fractions either at the pI stage or after the NPS analysis using conventional automation and multi-well plate fraction collectors and autosamplers without the need for complicated gel extractions. Typical analyses of whole cell lysates yield 1500 proteins with detection limits at or better than silver stained gels.

Eprogen jpeg

Eprogen, Inc. 8205 South Cass Ave., Suite 111, Darien, IL 60561 USA, Phone: 630.963.1481, Fax: 630.963.6432, www.eprogen.com

For more information please contact Joel Nott or Chu-Xiong Liao of the Protein Facility at 515-294-3267 (protein@iastate.edu) or Louisa Tabatabai at 515-294-6284 (lbt@iastate.edu)