Eprogen, Inc. is proud to introduce ProteoSep™ - a new and powerful protein
separation technology designed to more easily characterize and map complex protein mixtures.
It provides highly detailed maps of intact proteins in one day while
retaining the intact proteins in the liquid phase, allowing researchers the ability to more easily study
post translational modifications. The gel-free ProteoSep technology works with conventional HPLC equipment, directly interfacing with Mass Spectrometry and other characterization techniques.
Eprogen visited Iowa State on August 21, 2002, presenting ProteoSep to the Universitys Protein Facility. The following link to the presentation is a summary of the information that was presented at the seminar.
Visit Eprogen's website at www.eprogen.com for additional information on ProteoSep,
including protocols, and the following page in particular to view the variety of
samples that have been mapped using ProteoSep and this technology's wide-ranging applications:
http://www.eprogen.com/proteomics/literature/TechnicalPresentationForCD.pdf.
and
the Iowa State University Protein Facility
Present:
ProteoSep™:
An All-Liquid Phase Protein Mapping Technology...
the 1 Day Alternative to the 2D PAGE
Dr. Tim Barder
Mr. John Taulien
2:00 PM
Monday August 19, 2002
1102 Molecular Biology Building, Iowa State University
ProteoSep™ is a novel protein mapping technology and a powerful way to analyze complex
protein mixtures. ProteoSep is a gel-free, all-liquid phase alternative to 2D PAGE that
uses standard HPLC instrument technology to produce high resolution 2D maps of complex
protein systems such as whole cell lysates, plasma and sera. A unique high performance
chromatofocusing (CF) column has been developed to provide the pI information biologists
need in the first dimension analysis of complex protein systems. The use of HPLC provides
these pI fractions in the liquid phase containing the intact proteins. Subsequent analysis
of these pI fractions with proprietary reverse phase NPS® columns provides for the second
dimension separation information based on hydrophobicity or MW, depending on whether you
use UV or MS detection or both. A "2D protein map" is produced using ProteoVue™ Software,
which provides either UV - pI or Mass - pI maps or both, displaying the proteins present
in bands like that presented in a 2D PAGE gel. The all-liquid phase format provides for
easy collection of sample fractions either at the pI stage or after the NPS analysis using
conventional automation and multi-well plate fraction collectors and autosamplers without
the need for complicated gel extractions. Typical analyses of whole cell lysates yield
1500 proteins with detection limits at or better than silver stained gels.
For more information, please visit:
www.eprogen.com
Sign up by e-mailing: jtaulien@eprogen.com
Please include your e-mail address, full address, phone and fax numbers so that we can
keep you updated on new technologies we develop in the future.
8205 South Cass Ave., Suite 111
Darien, IL 60561
630.963.1481
www.eprogen.com