Electrospray Mass Spectrometry for Direct Analysis of lipid A from Enterobacter Agglomerans
&
LC/MS/MS: Principle and Applications
August 1st, 2003
2:30 pm
Gilman Hall room 1352
The introduction will focus on a general representation of mass spectrometry with the emphasis on electrospray ionization. ESI is widely used for high molecular weight thermolabile species such as proteins and peptides. Also, it allows efficient production of low molecular weight ions from drugs and drug metabolites.
The second part of the seminar will be about the direct analysis of lipid A from Enterobacter Agglomerans, a bacterium found in field cotton and believed to be the cause of Bissinosys, a respiratory disease that affects mill workers. Lipid A is a lipid component of lipopolysaccharides LPS which are found on the outer membrane of gram-negative bacteria. A comparison of positive and negative ion modes ESI data with a standard lipid A from Salmonella Minnesota led to a convincing structural identification of the unknown lipid A.
The latter part of the seminar will focus on the MS/MS techniques and their usefulness in structural identification of compounds. It is a powerful technique that could be used in analyzing products that are difficult to analyze with simple MS techniques. The focus will be given to the use of triple quadrupoles and the techniques that they can offer.
Kamel Harrata, Ph.D.
Mass Spectrometry Supervisor
www.public.iastate.edu/~kamel