In-gel digestion/peptide mass fingerprinting
The Investigator ProGest from Genomic Solutions is used for the automated in-gel digestion of protein samples from 1-D or 2-D gels. Gel spots can be digested with a variety of enzymes including trypsin, Arg-C and Glu-C. The resulting peptides from the digestion can then be analyzed by MALDI-TOF. From the spectrum obtained, peptide mass fingerprinting (using databases available on the WWW) can be performed to identify the original protein. The peptides from the digested proteins can also be separated by HPLC for further analysis by N-terminal sequencing or MALDI-TOF.
- Use the MALDI submission form (PDF) for in-gel digestion samples
- Instructions for database searching can be found in the manual for BBMB 542 Section E--Introduction to Proteomics, section 10E
- ProteinProspector, from UCSF, used for peptide mass fingerprinting
