Protein Facility

The Protein Facility of the Iowa State University Office of Biotechnology is open to faculty and students from the university, other educational institutions, and industry scientists. The facility offers protein/peptide sequencing, large and small scale peptide synthesis (Fmoc), matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, circular dichroism (CD), SDS-PAGE/blotting, 2-D gel electrophoresis, isoelectric focusing (IEF), in-gel digestion/peptide mass fingerprinting, digital image acquisition and analysis using the Typhoon imaging system and the 2D gel documentation/analysis system, and preparative, analytical and micro-analytical high performance liquid chromatography (HPLC).
MALDI, HPLC, SDS-PAGE/blotting, IEF, 2D gel electrophoresis, 2D gel documentation/analysis, the Typhoon imaging system and CD are all available for user operation after appropriate training.

• Circular Dichroism
• High Performance Liquid Chromatography (HPLC)
• MALDI Mass Analysis
• Peptide Synthesis
• Protein/Peptide Sequencing
• SDS-PAGE and Electroblotting
• 2-D gel electrophoresis
• Isoelectric focusing (IEF)
• In-gel digestion/mass fingerprinting
• Typhoon imaging system
• 2D gel documentation and analysis