Protein Facility

News

August 11, 2009
Facility acquires Progenesis SameSpots software from Nonlinear Dynamics for analysis of 2D gels

April 14, 2008
The amino acid analysis service has been discontinued, see here for details.

Common Matrices in MALDI-TOF

Matrix	MW (Da)	Applications preparation	Initial velocity (m/sec)^1,2	Characteristic monoisotopic matrix ions (in Da)
3,5-Dimethoxy-4-hydroxy-cinnamic acid (Sinapinic Acid) SA	224.07	Peptides Proteins 10 mg/mL in 70% ACN/water, 0.1% TFA	350	225.076 224.068 207.066 431.134
α-Cyano-4-hydroxycinnamic acid (ACH)	189.04	Peptides Proteins Saturated solution in 70% ACN/water, 0.1% TFA	300	164.047 195.050 172.040 379.093 212.032 294.076
2,5-Dihyroxybenzoic acid (DHB)	154.03	Peptides Carbohydrates Small molecules 10 mg/mL in 70% ACN/water, 0.1% TFA	500	155.034 154.027 137.024 273.040
3-Hydroxypicolinic acid (3HPA)	139.03	Oligonucleotides 9:1 dilution of matrix:diammonium citrate matrix: 50 mg/mL in 50% ACN/water Diammonium citrate: 50 mg/mL in water	550	96.045 140.035 279.062 235.072 234.064 233.056 191.082 189.066
Dithranol	226.06	Polymers 10 mg/mL in THF Add silver triflouroacetate to minimize Na+ and K+ adduct formation		225.055 226.063 227.071 211.076
2,2':5',2''-Terthiophene	248.39	Polymers 10 mg/mL in THF, mix 10:1 with analyte

ACN-acetonitrile
TFA-trifluoroacetic acid
THF-tetrahydrofuran

1. Juhasz, P., M. Vestal, and S.A. Martin, “On the Initial Velocity of Ions Generated by Matrix-Assisted Laser Desorption Ionization and Its Effect on the Calibration of Delayed Extraction Time-Of-Flight Mass Spectra”, J. Am. Soc. Mass. Spectrom., 1996, 8, 209-217.

2. Juhasz, P., M. Vestal, and S.A. Martin, “Novel Method for the Measurement of the Initial Velocity of Ions Genereated by MALDI”, presented at the 44^th ASMS Conference on Mass Spectrometry and Allied Topics, May 12-16, 1996, Portland, OR, p. 730.

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