February 20, 2009
iTRAQ: Application for the identification of vaccine candidates for swine and avian respiratory diseases
Mandy K. Zimmerli, Department of Biochemistry, Biophysics and Molecular Biology, Iowa State UniversityFriday February 20, 2009
11:10 AM-12:00 PM
1424 MBB
Abstract:
iTRAQ, which stands for isobaric tagging for relative and absolute quantification, is a method used to determine differential protein expression. One of the applications of this method is the identification of proteins that are up or down regulated in virulent organisms. The outer membrane proteins of various serotypes of Haemophilus parasuis, a swine respiratory pathogen, and Ornithobacterium rhinotracheale, an avian respiratory pathogen, were subjected to iTRAQ analysis in order to determine proteins that could serve as potential vaccine and diagnostic candidates.
