Protein Facility

The Protein Facility of the Iowa State University Office of Biotechnology is open to faculty and students from the university, other educational institutions, and industry scientists. The facility offers protein/peptide sequencing, large and small scale peptide synthesis (Fmoc), matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, circular dichroism (CD), SDS-PAGE/electroblotting, 2-D gel electrophoresis, isoelectric focusing (IEF), in-gel digestion/peptide mass fingerprinting, tandem mass spectrometry (LC-MS, LC-MS/MS and LC/LC-MS/MS), digital image acquisition and analysis using the Typhoon imaging system and the 2D gel documentation/analysis system, preparative, analytical and micro-analytical high performance liquid chromatography (HPLC) and analysis of biomolecular interactions using the Biacore T100 Surface Plasmon Resonance (SPR) instrument.
MALDI, HPLC, SDS-PAGE/blotting, IEF, 2D gel electrophoresis, the Biacore T100, 2D gel documentation/analysis, the Typhoon imaging system and CD are all available for user operation after appropriate training.

• Biacore T100 Surface Plasmon Resonance Instrument
• Circular Dichroism
• High Performance Liquid Chromatography (HPLC)
• MALDI Mass Analysis
• Peptide Synthesis
• Protein/Peptide Sequencing
• SDS-PAGE and Electroblotting
• 2-D gel electrophoresis
• Isoelectric focusing (IEF)
• Q-Star Tandem Mass Spectrometry
• In-gel digestion/mass fingerprinting
• Typhoon imaging system
• 1D and 2D gel documentation and analysis